It is distributed in various organs of the human body, including skin, hair, eyes, and brain ( Ko et al., 2014). Melanin is a pigment of skin which is synthesized by melanocytes in melanosomes. These results suggested that SPE could be used as a potential source of natural skin-whitening material in cosmetics as well as in food products. In particular, quercetin exhibited similar inhibitory activities against tyrosinase and L-DOPA oxidation without cytotoxicity. Of the compounds isolated, gallic acid and quercetin revealed significant inhibitory activities against melanogenesis compared to arbutin. A cytotoxic activity assay was done to examine the cellular toxicity in Raw 264.7 macrophage cells. The whitening effect of the isolated compounds from SPEE were tested for the inhibitory activities against melanogenesis using B16 melanoma cells, in vitro inhibition of tyrosinase, and L-3,4-dihydorxy-indole-2-carboxylic acid (L-DOPA) auto-oxidation assay. By bioassay-guided isolation, ten phenolic compounds were isolated by column chromatography from SPEE. SPE and SPEE showed significant inhibitory effects against melanogenesis and tyrosinase activities. pseudocamellia were extracted with hot water (SPE) and partitioned with n-hexane (SPEH), dichloromethane (SPED), and ethyl acetate (SPEE) successively.
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